✦ LIBER ✦

Effect of ethylene glycol-bis(β-aminoethyl ether)-N,N-tetraacetic acid (EGTA) on stability and activity of methanogenic granular sludge

✍ Scribed by J. T. C. Grotenhuis; J. B. Lier; C. M. Plugge; A. J. M. Stams; A. J. B. Zehnder

Publisher: Springer
Year: 1991
Tongue: English
Weight: 934 KB
Volume: 36
Category: Article
ISSN: 1432-0614
DOI: 10.1007/bf00164709

No coin nor oath required. For personal study only.

✦ Synopsis

The effect of the calcium-specific chelant ethylene glycol-bis(fl-aminoethyl ether)-N,N-tetraacetic acid (EGTA) on methanogenic granular sludge from a laboratory-scale upflow anaerobic sludge-blanket (UASB) reactor fed propionate and from a full-scale reactor treating paper-mill waste-water was studied. Upon treatment with EGTA both sludge types showed a decrease in the calcium and phosphorus content and a release of protein and polysaccharides, leading to a decrease in strength of papermill granular sludge and a disintegration of propionate-grown granules. After treatment of propionate-grown granular sludge with high EGTA concentrations, the methanogenic activity with propionate and acetate as test substrates decreased by 88 and 33%, respectively. The marked reduction in propionate oxidation activity may be caused by a disruption of the special juxtapositioning of bacteria in the granules.

📜 SIMILAR VOLUMES

A test to evaluate the effect of individ

A test to evaluate the effect of individual components of ethylene glycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid buffers on enzymatic activity

✍ Hubert Vidal; Jean-Francois St-Denis; Edith Painchaud; Marie Josée Larue; Gérald 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 537 KB

A simple dilution test for evaluating the individual effect on enzymatic activity of [Ca2+], [EGTA], or [Ca.EGTA] variations in Ca-EGTA buffers is presented. We verified that a 50-fold dilution of the buffer (25-0.5 mM) at constant pH did not affect [Ca2+] (measured with fura-2), whereas [EGTA] and

A stopped-flow investigation of calcium

A stopped-flow investigation of calcium ion binding by ethylene glycol bis(β-aminoethyl ether)-N,N′-tetraacetic acid

✍ Paul D. Smith; Gerald W. Liesegang; Robert L. Berger; George Czerlinski; Richard 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 761 KB

The kinetics of calcium ion complexation by ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) were investigated using the stopped-flow technique. This study was performed within the pH range 5.8 to 8.4. The reaction was found to be first order in EGTA and complex order in calci