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Effect of Ethanol and Chlorpromazine on Transhepatic Transport and Biliary Secretion of Horseradish Peroxidase

✍ Scribed by Takeshi Okanoue; Isao Kondo; Thomas J. Ihrig; Samuel W. French

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
965 KB
Volume
4
Category
Article
ISSN
0270-9139

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✦ Synopsis

In order to demonstrate the effect to the acute administration of ethanol and chlorpromazine (CPZ) on bile flow and transhepatic transport of horseradish peroxidase (HRP) into bile, male rats were administered either 5 gm per kg ethanol intragastrically (E-rats) or 3 mg per kg CPZ intraperitoneally (CPZ rats). Control rats (C-rats) received saline. Two hours after ethanol feeding or 90 min after CPZ injection HRP was injected into the portal vein, and bile samples were collected at 10-min intervals for 2 hr. Tissue samples were removed at 1, 10,60, and 120 min to study HRP transport using electron microscopic cytochemical localization. Bile flow was reduced (p c 0.001) both in E-and CPZ-rats compared to C-rats. In E-rats HRP secretion was significantly decreased at 30 and 40 min post-HRP injection (p < 0.05) and the peak rate of HRP secretion was delayed

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