Effect of enzyme concentration on apparent specific activity of hydrogenase

✍ Scribed by Andrá Dér; Csaba Bagyinka; Tibor Páli; Kornél L. Kovács

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 462 KB
• Volume: 150
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90538-x

✦ Synopsis

The effect of enzyme concentration on the Ha-uptake and HZ-evolving activities of the reversible hydrogenase from Thiocapsa roseopersicina was examined. In the activity range assayed by a spectrophotometric technique the apparent Hz-uptake specific activity varied greatly with hydrogenase concentration. Study of Hz-evolving activity measured by the Hz electrode method and compared with a gas chromatographic assay also indicated that specific activity was highly dependent on enzyme concentration. The results indicate that the widely applied hydrogenase assays give systematically erroneous specific activity values. These assays should be used only for relative measurements and the hydrogenase concentration in the reaction mixture should be kept constant. To make the data from various laboratories comparable the assay parameters should be standardized. 0 1985 Academic press, IIIC.