Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures
β Scribed by Sushil K. Rijhwani; Jacqueline V. Shanks
- Book ID
- 109388104
- Publisher
- American Institute of Chemical Engineers
- Year
- 1998
- Tongue
- English
- Weight
- 136 KB
- Volume
- 14
- Category
- Article
- ISSN
- 8756-7938
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Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by
## Abstract Nitric oxide (NO) is known as a signaling molecule involved in elicitorβinduced defense responses of plants. Sodium nitroprusside (SNP), a donor of NO, stimulates catharanthine formation in Catharanthus roseus cells.1 Two important terpenoid indole alkaloids produced in small quantities
Cultures of C. roseus transgenic (''hairy'') root clones LBE-6-1 and LBE-4-2 were adapted with periodic daily illumination to investigate the effect of light on growth and nutrient utilization, and the accumulation of the indole alkaloids. Light-adapted roots appeared green and had radially thickene