✦ LIBER ✦

Effect of electric currents on DNA synthesis in rat osteosarcoma cells: Dependence on conditions that influence cell growth

✍ Scribed by Masaki Noda; David E. Johnson; Allesandro Chiabrera; Dr. Gideon A. Rodan

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 769 KB
Volume: 5
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.1100050212

No coin nor oath required. For personal study only.

✦ Synopsis

Effects of sinusoidal 60-Hz electric currents on DNA synthesis in rat osteosarcoma cell (ROS 17/2.8) in culture were investigated using agar electrodes. This exposure system eliminates electrochemical effects at electrode surfaces, minimizes the contribution of magnetic fields and produces no detectable temperature changes (< t 0. l°C) at current densities of up to 1,500 pA r.m.s. (root mean square)/cm2. Thirty-four hours exposure to electric current at intensities of 300-400 pA r.m.s./cm2 enhanced DNA synthesis by 20% relative to controls. The effect depended on serum concentration, cell seeding density, and the age of the cell population at the time of seeding: effects were seen at 0.2% fetal bovine serum, in cells seeded 4 days after previous passage, at 20,000 cells/cm2. These findings indicate that sinusoidal electric currents can mildly enhance DNA synthesis in these cells under growth-limiting conditions.

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