𝔖 Bobbio Scriptorium
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Effect of egg water from Bufo arenarum on the fertilizing capacity of homologous spermatozoa

✍ Scribed by Fontdevila, Marina F. Díaz ;Bloj, Bernabé ;Cabada, Marcelo O.

Book ID
102894177
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
645 KB
Volume
257
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The effect of egg water (EW) from oocytes' string of Bufo arenarum on the fertilizing capacity of homologous spermatozoa was studied. When Bufo arenarum spermatozoa were incubated in EW, a decrease in the fertilizing capacity of spermatozoa was observed. This effect depended on the osmolarity of the incubation medium. When the osmolarity was between 35 and 40 mOsm/kg water, inhibition of fertility was observed even in the presence of only 10% EW. When osmolarity was greater than 60 mOsm/kg water, even 90% EW had no effect. Control spermatozoa incubated in saline medium with osmolarity similar to that of EW did not show any decrease in fertility. These effects seem to be related to a component of the EW that can be extracted with agents used to extract lipids. The effect of such lipidic extracts of egg water (LEEW) in reducing the fertilizing capacity of a sperm suspension depends on the concentration of LEEW and the duration of incubation.

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