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Effect of dihydrotestosterone on turnover of alcohol dehydrogenase in rat hepatocyte culture

✍ Scribed by Esteban Mezey; Lynda Rennie-Tankersley; James J. Potter

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 117 KB
Volume: 27
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.510270129

No coin nor oath required. For personal study only.

✦ Synopsis

Animals and Chemicals. Male Sprague-Dawley rats were obtained from Charles River Laboratories (Wilmington, MA). All animals received humane care in compliance with the guidelines from the Animal Care and Use Committee of the Johns Hopkins University. Dulbecco' s modified Eagle' s medium (DMEM), fetal bovine serum (FBS), penicillin-steptomycin, cesium chloride, and guanidinium isothiocyanate were purchased from Life Technologies (Gaithersburg, MD). Leucine-free DMEM, collagenase (type IV), Trypan Blue, bovine serum albumin (BSA), fetuin, transferrin, linoleic acid, sodium selenite, and dexamethasone were purchased from Sigma Chemical Company (St. Louis, MO).

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