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Effect of culture period and cell density on regrowth following cryopreservation of embryogenic suspension cultures of Norway Spruce and Sitka spruce

✍ Scribed by Jens I. Find; Michel M.H. Kristensen; Jens V. Nørgaard; Peter Krogstrup


Book ID
110231812
Publisher
Springer
Year
1998
Tongue
English
Weight
203 KB
Volume
53
Category
Article
ISSN
0167-6857

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Reflexed saltmarsh-grass suspension cultures produced by seed callus were frozen to the liquid nitrogen temperature. Cooling rates, cryoprotectants and holding times were taken as a function of transfer temperatures. The highest survival of cells (45%) was found at a freezing rate of I°C rain -I , w