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Effect of culture conditions on glutathione content in A549 cells

✍ Scribed by Gloria B. Post; Douglas A. Keller; Karen A. Connor; D.B. Menzel

Book ID
115756641
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
352 KB
Volume
114
Category
Article
ISSN
0006-291X

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✦ Synopsis

Summarv: The effects of varying culture conditions on glutathione content in A549 (human type II lung tumor derived) cells were examined. Parameters studied were growth time, serum concentration, and the presence or absence of a mixture of insulin, transferrin, and selenous acid. Glutathione content increased with serum concentration.

When cells were grown with serum, glutathione increased sharply 24 hours after passage and decreased thereafter. Insulin, transferrin, and selenous acid had little effect on cell growth or glutathione content.

Replacement of media with fresh media containing 10% serum did not prevent the growth dependent decrease in glutathione.

These results demonstrate that glutathione content in A549 cells is strongly affected by culture conditions.

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✍ Y. James Kang; Yi Feng; Emiko L. Hatcher πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 964 KB

Extracellular glutathione (GSH) is degraded by an external cell-surface enzyme, y-glutamyltranspeptidase (y-GT). The products are transported into cells to participate in important cellular processes. In the present study, we tested the hypothesis that extracellular GSH is a source of glutamic acid