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Effect of copy number and mRNA processing and stabilization on transcript and protein levels from an engineered dual-gene operon

โœ Scribed by Christina D. Smolke; Jay D. Keasling


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
434 KB
Volume
78
Category
Article
ISSN
0006-3592

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โœ Trent Carrier; Kristala L. Jones; J. D. Keasling ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 179 KB

The effects of mRNA stability and plasmid copy number on gene expression in Escherichia coli were evaluated by constructing multicopy (pMB1-based) and low-copy (F-based) plasmids containing an arabinoseinducible promoter system, the lacZ reporter gene, and mRNA-stabilizing 5ะˆ hairpin structures. Pro