Effect of continuous darkness on diurnal rhythm in glycogen content in pineal cells of the mouse: A semiquantitative histochemical study
✍ Scribed by Kachi, Takashi ;Matsushima, Shoji ;Ito, Takashi
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 444 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0003-276X
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✦ Synopsis
Abstract
The effect of continuous darkness on diurnal variation of glycogen content in pineal cells of adult mice was investigated by means of a semiquantitative histochemical method. In dark‐maintained mice, a diurnal rhythm in pineal glycogen content persisted for the first 7 days of darkness. After 14 days or longer of continuous darkness, however, a clear relationship between the glycogen content and time of day became less apparent. Reversed conditions of environmental lighting caused a complete reversal of the glycogen rhythm in the pineal as early as the fifth day. When mice that had been kept in reversed conditions of lighting for 7 days were exposed to continuous darkness, the reversed glycogen rhythm also persisted in darkness of 7 and 14 days, but it was abolished by darkness of 28 days. The nuclear density of pineal cells was significantly lower at 9 am than at 9 pm in controls. In dark‐maintained mice, however, no significant diurnal variation in pineal cell size was generally evident except 2 and 3 days after the initiation of darkness.