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Effect of CO2 and CHF3 on the Solubilization of Protein in Reverse Micelles

✍ Scribed by Haifei Zhang; Buxing Han; Guanying Yang; Haike Yan

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 53 KB
Volume: 232
Category: Article
ISSN: 0021-9797
DOI: 10.1006/jcis.2000.7214

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✦ Synopsis

The effect of dissolved CO 2 and CHF 3 on the precipitation of bovine serum albumin (BSA) from reverse micelles of sodium bis(2ethylhexyl) sulfosuccinate (AOT) in 2,2,4-trimethylpentane (isooctane) has been studied at 308.15 K. It was found that BSA can be precipitated completely by CO 2 while the AOT and water remain in the iso-octane continuous phase. This opens up a possibility for recovery of protein solids directly from reverse micellar solutions. CHF 3 is also an effective agent for precipitation of BSA in the reverse micelles when the expansion coefficient of CHF 3 exceeds 0.4. However, the condition required for complete removal of the BSA using CHF 3 results in some precipitation of AOT and water from the solution.

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