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Effect of charge derivatization in the determination of phosphorylation sites in peptides by electrospray ionization collision-activated dissociation tandem mass spectrometry

✍ Scribed by Sadagopan, Nalini; Malone, Michael; Watson, J. Th-rock

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 72 KB
Volume: 34
Category: Article
ISSN: 1076-5174
DOI: 10.1002/(sici)1096-9888(199912)34:12<1279::aid-jms899>3.0.co;2-9

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✦ Synopsis

Phosphorylation is a post-translational modification of proteins important for signal transduction and enzyme activity. Electrospray ionization mass spectrometry (ESI-MS) has served as an important tool in the identification of phosphorylation sites in many proteins. Enzymatic digestion and peptide mass mapping have been routinely used for this purpose, 1 -4 and MS/MS with collision-activated dissociation (CAD) has been employed to obtain unambiguous results. 5 -12 CAD-MS/MS of a peptide often yields a variety of fragment ions retaining either the N-

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