Effect of Chain Density on Inhibition of Protein Adsorption by Poly(ethylene glycol) Based Coatings

✍ Scribed by Martin Malmsten; Ken Emoto; James M. Van Alstine

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 220 KB
• Volume: 202
• Category: Article
• ISSN: 0021-9797
• DOI: 10.1006/jcis.1998.5513

✦ Synopsis

of an essentially steric repulsion and the simultaneous ab-The effect of interfacial chain density of poly(ethylene glycol) sence of strong attractive interactions between a wide range (PEG) on the adsorption of serum proteins was investigated by of proteins and PEG-containing coatings, appear to be the in situ ellipsometry. For terminal covalently grafted PEG of molecmost important.

ular weight 5000 an increased grafting density results in decreased

The protein rejecting capability of PEG-containing coatserum protein adsorption. At high interfacial chain density (É0.1 ings is expected to depend on a range of parameters, includchain/nm 2 ), efficient protein rejection was observed, irrespective ing PEG molecular weight, interfacial PEG chain density, of the coupling chemistry used. Strongly adsorbed PEG-containing polymer chain architecture, etc. Of particular interest to the polymers behaved similarly to covalently attached PEG regarding present investigation are the effects of PEG grafting density inhibition of protein adsorption, independent of the nature of the underlying surface. The results are discussed in relation to the on the inhibition of protein adsorption. The reason for this importance of the protein size in protein rejection by PEG coatings. is that, contrary to the molecular weight dependence, the