𝔖 Bobbio Scriptorium
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Effect of cellobiose, glucose, ethanol, and metal ions on the cellulase enzyme complex of Thermomonospora fusca

✍ Scribed by John D. Ferchak; E. Kendall Pye

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
377 KB
Volume
25
Category
Article
ISSN
0006-3592

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✦ Synopsis

The saccharification of cellulosic substrates by cellulase from Thermomonospora fusca, strain YX, was influenced by the presence of various substances. Cellobiose was strongly inhibitory, reducing the activity against swollen cellulose to 25% at 5% concentration. Glucose had much less effect, reducing activity to 40% at 20% concentration. Ethanol was found to be only slightly inhibitory to the cellulase, reducing activity by about 15% at 6% concentration, but much more inhibitory to the cell-bound P-glucosidase activity. Of the numerous metal ions examined, Ca" and Coz+ at 0 . 1 m M -1 . W concentration were found to be slightly activating under assay conditions, while 1 . W Pb2+ and Hg'+ were the most inhibitory ions. The implications of these results for the design of commercial processes for ethanol production from cellulose are discussed.

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