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Effect of carbon-substrates on biomass growth and recombinant protein formation in continuous cultures of Pichia pastoris Mut+ strain

✍ Scribed by L. Paulova; M. Jan; K. Melzoch; J. Klein; H.P. Meyer; K. Kovar


Book ID
108217209
Publisher
Elsevier
Year
2009
Tongue
English
Weight
85 KB
Volume
25
Category
Article
ISSN
1871-6784

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Continuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic analysis of growth and product formation. Using methanol as the sole source of carbon, energy, and induction, cell growth could