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Effect of calcium, manganese and lithium on growth and cardenolide content in cell suspension cultures ofDigitalis thapsiL.

✍ Scribed by M. Purificación Corchete; M. Amparo Jimenez; Margarita Moran; Margarita Cacho; Jorge Fernandez-Tarrago

Publisher
Springer
Year
1991
Tongue
English
Weight
279 KB
Volume
10
Category
Article
ISSN
0721-7714

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✦ Synopsis

Cell suspension cultures of Digitalis thapsi were grown in Murashige and Skoog medium under continuous light. The effects of the absence of CaCl2, elevation of the MnSO4 concentration from 0.1 mM to 5 mM or the addition of 100 μM LiCl on their growth and digoxin production were investigated. The elimination of calcium reduced growth and viability of cultures but promoted digoxin formation. An increase of the MnSO4 concentration or the addition of LiCl resulted in higher digoxin content. Under such conditions growth was not affected.

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