✦ LIBER ✦

Effect of butylated hydroxytoluene on biliineage differentiation of the human HL-60 myeioblastic leukemia cell

✍ Scribed by C. Patrick Burns; Eric S. Petersen

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 637 KB
Volume: 144
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041440106

No coin nor oath required. For personal study only.

✦ Synopsis

City, lowa 52242

Butylated hydroxytoluene (BHT), which has both antioxidant and membrane active properties, has been reported to affect cellular differentiation. We studied its etfect on the bipolent lineage differentiation of the important HL-60 human myeloblastic leukemia cell line using reduction of nitroblue tetrazolium, cell cycle analysis, population growth rate, monoclonal antibodies, and morphology. BHT markedly accelerated retinoic acid-induced myelocytic differentiation and dihydroxyvitamin D,-induced monocytic differentiation in a concentration and timedependent manner. Butylated hydroxyanisole (BHA) had a comparable effect.

Preincubation with the compounds was not necesary to evoke the acceleration.

Other antioxidants and inhibitors of eicosanoid synthesis were inactive. We conclude that the important food preservatives BHT and BHA accelerate the kinetics of terminal differentiation of human leukemia and that this effect is likely due at least in part to their membrane active properties.

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