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Effect of buffer composition on the migration order and separation of histone H1 subtypes

✍ Scribed by Prof. Herbert Lindner; Wilfried Helliger; Bettina Sarg; Christoph Meraner

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
663 KB
Volume
16
Category
Article
ISSN
0173-0835

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✦ Synopsis

Abstract

The effects of different buffer concentrations and compositions on the elution order and separation of H1 histone subtypes and their phosphorylated modifications isolated from several species was studied using high‐performance capillary electrophoresis (CE). Various cations and anions were tested in an untreated silica capillary and low pH buffers, in the presence of the dynamic coating agent hydroxypropylmethyl cellulose. It was found that the cations and anions of buffers have a remarkable influence on both the efficiency and the selectivity of protein separations. A triethylammonium methanephosphonate system proved efficacious for the separation of rat histone subtype H1c from H1e and a perchlorate/triethylammonium phosphate system for the analysis of chicken and mouse linker histones. CE provides an attractive alternative to high‐performance liquid chromatography and conventional gel electrophoresis.

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