Effect of bovine serum albumin on particulate methane monooxygenase from Methylosinus trichosporium OB3b

✍ Scribed by Masayuki Takeguchi; Ichiro Okura

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 414 KB
• Volume: 145
• Category: Article
• ISSN: 1381-1169
• DOI: 10.1016/s1381-1169(99)00010-2

✦ Synopsis

The effect of some additives on the membrane bound particulate methane monooxygenase pMMO from Methylosinus trichosporium OB3b was studied. The pMMO activity was stimulated by exogenously supplied bovine serum albumin Ž . Ž . BSA , but was inhibited by ovalbumin and excess copper II ion. When the membrane was treated with BSA, the amount Ž . of copper II ion in the membrane decreased, but the specific activity increased about 1.8 times compared with the Ž . as-isolated membrane. BSA may remove excess copper II ions in the membrane, inhibiting the pMMO activity. When the excess copper is removed by BSA, pMMO activity is stimulated.