𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of benzyl alcohol on recombinant human interleukin-1 receptor antagonist structure and hydrogen–deuterium exchange

✍ Scribed by John R. Alford; Andrew C. Fowler; Deborah S. Wuttke; Bruce A. Kerwin; Ramil F. Latypov; John F. Carpenter; Theodore W. Randolph

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
505 KB
Volume
100
Category
Article
ISSN
0022-3549

No coin nor oath required. For personal study only.

✦ Synopsis

Benzyl alcohol, a preservative commonly added to multidose therapeutic protein formulations, can accelerate aggregation of recombinant human interleukin-1 receptor antagonist (rhIL-1ra). To investigate the interactions between benzyl alcohol and rhIL-1ra, we used nuclear magnetic resonance to observe the effect of benzyl alcohol on the chemical shifts of amide resonances of rhIL-1ra and to measure hydrogen-deuterium exchange rates of individual rhIL-1ra residues. Addition of 0.9% benzyl alcohol caused significant chemical shifts of amide resonances for residues 90-97, suggesting that these solvent-exposed residues participate in the binding of benzyl alcohol. In contrast, little perturbation of exchange rates was observed in the presence of either sucrose or benzyl alcohol.

📜 SIMILAR VOLUMES

Effects of benzyl alcohol on aggregation
Effects of benzyl alcohol on aggregation of recombinant human interleukin-1-receptor antagonist in reconstituted lyophilized formulations
✍ Shouvik Roy; Rose Jung; Bruce A. Kerwin; Theodore W. Randolph; John F. Carpenter 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB

A major limitation in the successful development of multidose protein formulations is protein aggregation induced by antimicrobial preservatives such as benzyl alcohol, which are included to maintain product sterility. Studies were conducted to evaluate the strategy of developing lyophilized formula

Mechanism for benzyl alcohol-induced agg
Mechanism for benzyl alcohol-induced aggregation of recombinant human interleukin-1 receptor antagonist in aqueous solution
✍ Ye Zhang; Shouvik Roy; Latoya S. Jones; Sampathkumar Krishnan; Bruce A. Kerwin; 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 200 KB

Benzyl alcohol, an antimicrobial preservative, accelerates aggregation and precipitation of recombinant human interleukin-1 receptor antagonist (rhIL-1ra) in aqueous solution. The loss of native monomer during incubation at 378C was determined by analysis of sample aliquots with size exclusion high