Effect of basic fibroblast growth factor and α-melanocytic stimulating hormone on nerve regeneration through a collagen channel

✍ Scribed by Dr. Annie Laquerriere; Pascal Peulve; Olivier Jin; Jérôme Tiollier; Michel Tardy; Hubert Vaudry; Jacques Hemet; Marc Tadie

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 716 KB
• Volume: 15
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/micr.1920150312

✦ Synopsis

Abstract

An experimental study on the rat sciatic nerve was performed to evaluate nerve regeneration through a collagen guide and to study the effects of α‐melanocytic stimulating hormone (α‐MSH) and basic fibroblast growth factor (b‐FGF) in accel erating axonal elongation. After transection, nerves were repaired over a 7 mm gap using a placental collagen type IV guide. The channel was filled with either a b‐FGF solution or an α‐MSH solution or was produced with b‐FGF incorporated into the guide. Four weeks later, only groups in which b‐FGF had been injected or incorporated displayed a significant somatosensory evoked potential response. Histological and quantitative analysis of nerve fibres confirmed the existence of nerve continuity in groups receiving an α‐MSH solution or a channel containing b‐FGF. These results demonstrate that α‐MSH in solution and b‐FGF incorporated into a collagen type IV channel enhance peripheral nerve regeneration. However, at 4 weeks, only b‐FGF (3 ng) restores functional activity.