Effect of abscisic acid, osmolarity and temperature onin vitrodevelopment of recalcitrant mango nucellar embryos

✍ Scribed by Fernando Pliego-Alfaro; Richard E. Litz; Pamela A. Moon; Dennis J. Gray

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 648 KB
• Volume: 44
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00045913

✦ Synopsis

Development of cotyledonary-stage nucellar embryos of mango was arrested in vitro by exposure to 750-1750 #M ABA. The enlargement and germination of nucellar embryos was inhibited for as long as 4 weeks after subculture from ABA-containing medium. Mannitol at concentrations between 7.5 and 12.5% inhibited nucellar embryo development, presumably due to osmotic effects; however, there was no residual effect after subculture of somatic embryos onto medium without mannitol. Temperatures between 22.5 and 37.5 °C stimulated embryo development, whereas lower temperatures (7.5 and 15 °C) delayed germination. There was no germination 1 month after somatic embryos, pulsed for 8 weeks at 7.5 °C, were transferred to 22.5 °C; however, after 2 months, 86% of these somatic embryos germinated. These results indicate that it is possible to induce developmental arrest in recalcitrant mango embryos with high concentrations of ABA, mannitol or low temperature (7.5 °C).