Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli
โ Scribed by Khandaker Al Zaid Siddiquee; Marcos J. Arauzo-Bravo; Kazuyuki Shimizu
- Book ID
- 109327858
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 469 KB
- Volume
- 235
- Category
- Article
- ISSN
- 0378-1097
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In a previous study, an Escherichia coli strain lacking the key enzymes (acetate kinase and phosphotransacetylase, ACK-PTA) of the major acetate synthesis pathways reduced acetate accumulation. The ackA-pta mutant strain also exhibits an increased lactate synthesis rate. Metabolic flux analysis sugg
## Abstract The RNA polymerase sigma factor, encoded by __rpo__S gene, controls the expression of a large number of genes in __Escherichia coli__ under stress conditions. The present study investigated the growth characteristics and metabolic pathways of __rpo__S gene knockout mutant of __E. coli__