Effect of a confocal pinhole in two-phot
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Gauderon, R.; Lukins, P. B.; Sheppard, C. J. R.
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Article
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1999
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John Wiley and Sons
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English
⚖ 300 KB
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We investigated the effect of a finite-sized confocal pinhole on the performance of nonlinear optical microscopes based on two-photon excited fluorescence and second-harmonic generation. These techniques were implemented using a modified inverted commercial confocal microscope coupled to a femtoseco