Effect of 6-hydroxydopamine on in vitro hippocampal corticosterone binding capacity in the male rat

✍ Scribed by J. Weidenfeld; R. A. Siegel; A. P. Corcos; M. Chen; S. Feldman; I. Chowers

• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 378 KB
• Volume: 52
• Category: Article
• ISSN: 0014-4819
• DOI: 10.1007/bf00237156

✦ Synopsis

Adult male rats were injected with 6-hydroxydopamine, either into the lateral brain ventricle or directly into the dorsal hippocampus. They were adrenalectomized 5-7 days later, and following an additional 24 hours, the specific in vitro 3H-corticosterone binding capacity of dorsal hippocampal slices was determined by estimation of uptake of radioactivity by the nuclear fraction. Specific corticosterone binding was reduced by 40-50% in both experimental groups, as compared to vehicle-treated controls. These results suggest that the maintenance of normal dorsal hippocampal corticosterone binding capacity is dependent upon the integrity of endogenous brain catecholaminergic neural systems.