Effect of 3-methylcholanthrene and dimethylnitrosamine on anchorage dependence of rat fibroblasts chronically infected with rauscher leukemia virus

✍ Scribed by Nirmal K. Mishra; Wayne L. Ryan

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: French
• Weight: 579 KB
• Volume: 11
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910110114

✦ Synopsis

Abstract

This report describes the loss of anchorage dependence (AD–) after treatment with 3‐methylcholanthrene or dimethylnitrosamine in rat embryo fibroblasts which normally exhibit anchorage dependence (AD+). The AD+ fibroblasts which were chronically infected with Rauscher leukemia virus showed the highest frequency of transformation to AD– cells with chemical carcinogen treatment. A small percentage of AD– cells also appear in uninfected control cells treated with dimethylnitrosamine or at much higher frequency with 3‐methylcholanthrene. The virally infected cells also showed a detectable level of transformation to AD– cells without added chemicals. However, the combined effects of Rauscher leukemia virus infection and chemical carcinogen treatment were much greater than the additive effect of the two. No clear correlation between AD– transformation and the ability to grow in factor‐free medium was found. The significance of these observations is discussed in relation to the “oncogene” hypothesis.