Effect fixation on T and B lymphocyte surface membrane antigen demonstration in paraffin processed tissue
✍ Scribed by Dr Clive S. Holgate; Peter Jackson; Kenneth Pollard; Declan Lunny; Colin C. Bird
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 890 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0022-3417
No coin nor oath required. For personal study only.
✦ Synopsis
The identification of lymphoid surface membrane antigens in tissue sections using immunohistochemical techniques is becoming increasingly important for the diagnosis and classification of lymphoproliferative disorders. Many of the lymphocyte specific monoclonal antibodies used, however, can only be applied to frozen tissue sections. In this paper we report the successful application of a number of these antibodies to paraffin processed tissue utilizing alternative fixatives and the highly sensitive immunogold-silver staining method. The best fixatives for this purpose were formol dichromate, periodate-lysine-paraformaldehyde (PLP) and a novel fixative formed from the addition of a dichromate solution to PLP.