Ecological and physiological studies on thermophilic bacilli from sulfataric hot springs of central Italy
✍ Scribed by Francesco Canganella; Luigi Daniele Trovatelli
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 738 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Hot springs located in a geothermal area of central Italy were investigated with respect to the total number of thermophilic microorganisms as well as to the presence of amylolytic and pullulolytic bacteria. A number of motile thermophilic spore-forming bacteria were isolated from different sites. The aerobic organisms grew optimally at 60-65 "C on yeast extract-trypticase and/or xylose-starch supplemented mineral medium and spores were mostly observed on agar plates after prolonged incubation. According to the results based on phenotypic characterization, G+C% and lipid analyses all the aerobic isolates belong to the genus Bacillus and are characterized by an unusual G + C content ranging between 33.4-338.8. A limited number of anaerobic organisms were also isolated and investigated for the presence of amylolytic and pullulolytic activity. According to physiological tests these isolates were identified as Clostridium thermohydrosulfuricum; when grown on starch, they produced thermostable a-amylases, pullulanases and a-glucosidases.