Ecdysteroid induction of cell surface contacts in aDrosophila cell line

✍ Scribed by Yudin, Ashley I. ;Clark, Wallis H. ;Chang, Ernest S.

• Book ID: 102336933
• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 466 KB
• Volume: 219
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402190318

✦ Synopsis

Abstract

Drosophila Kc cells are usually spherical in shape and are relatively free of cell‐cell contacts. Soon after exposure to 20‐hydroxyecdysone they develop extensions, assume a fibroblastic shape, and closely adhere to adjacent cells by means of cell‐cell contacts to form large multicellular aggregates. This adhesive nature is enhanced with trypsin treatment without disruption of cell shape. Simultaneous addition of 20‐hydroxyecdysone and juvenile hormone results in diminished aggregate formation without preventing the changes in cell shape. These factors point to the possibility that 20‐hydroxyecdysone stimulates modifications in the cell surface that may be important during embryological differentiation.