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EBV infection of mitogen-stimulated human B lymphocytes

✍ Scribed by Lena Einhorn; Eva Klein

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
275 KB
Volume
27
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

B lymphocytes from human blood were treated with the mitogen Protein A (Staph. aureus), which induces DNA synthesis in, as well as differentiation of, the B lymphocytes. The cells were subsequently exposed to Epstein‐Barr virus (EBV). EBV‐infected and mitogen‐stimulated cells were detected simultaneously, by using a combination of immunofluorescence (for EBNA) and autoradiography (for DNA‐synthesis). In the initial phase (1‐2 days) after addition of the mitogen, stimulated cells were as susceptible to infection as resting cells. Thereafter, their susceptibility decreased. This suggests that, although initiation of DNA‐synthesis does not seem to limit sensitivity to the viral infection, differentiation of the B cells might do so. The intensity and pattern of EBNA‐staining in prestimulated cells differed from that of the controls.

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