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Easy determination of ploidy level inArabidopsis thalianaplants by means of pollen size measurement

โœ Scribed by Thomas Altmann; Brigitte Damm; Wolf B. Frommer; Thomas Martin; Peter C. Morris; Dieter Schweizer; Lothar Willmitzer; Renate Schmidt

Publisher
Springer
Year
1994
Tongue
English
Weight
857 KB
Volume
13
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.

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