𝔖 Bobbio Scriptorium
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Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis

✍ Scribed by Lily X. Zelaya-Molina; Maria A. Ortega; Anne E. Dorrance

Book ID
106364972
Publisher
Springer Netherlands
Year
2010
Tongue
English
Weight
229 KB
Volume
33
Category
Article
ISSN
0141-5492

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## Abstract Fruit tissues are considered recalcitrant plant tissue for proteomic analysis. Three phenol‐free protein extraction procedures for 2‐DE were compared and evaluated on apple fruit proteins. Incorporation of hot SDS buffer, extraction with TCA/acetone precipitation was found to be the mos