Early onset of serum blocking in a murine melanoma model

✍ Scribed by Dr. Alan E. Bray; Patrick G. Holt; Lynne M. Roberts; David Keast

• Book ID: 102863281
• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: French
• Weight: 638 KB
• Volume: 16
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910160410

✦ Synopsis

Abstract

The early onset of serum blocking of host cell‐mediated immunity was investigated in a transplantable murine melanoma model employing two in vitro assays. One of these was based on the Takasugi and Klein cytotoxicity test; the other was a new test based on the inhibition of adherence of leukocytes to a plastic surface in the presence of tumor antigen. Both tests yielded similar results, and the adherence inhibition test was more sensitive. Blocking could be detected as early as 1 h after the inoculation of 1×10^7^ melanoma cells. Early onset of serum blocking was also demonstrable if homogenized or tissue‐cultured tumor cells were inoculated. Serum blocking under these circumstances was initially transient, declining at 24 to 48 h, but reappearing by the 4th day after inoculation. Furthermore, a functional immune system was not required for blocking factor development in this system, as evidenced by the onset of a similar pattern of blocking activity in heavily irradiated animals.