Early metabolic changes in regenerating microfragments of the liverwortRiella helicophylla(Bory et Mont.) Mont.: Protein and RNA synthesis, free α-amino concentration
✍ Scribed by Burckhard Viell
- Book ID
- 104754301
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 557 KB
- Volume
- 137
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Microfragments of constant size were isolated from the thallus of Riella by a rapid punching procedure. Thus it was possible to study various metabolic parameters especially during the first hours after fragment isolation. Protein synthesis increases rapidly after a slight decrease at 30 min. The earliest significant increase in RNA synthesis occurs at 8 h, indicating a different activation pattern. The concentration of e-amino compounds drops at 30 rain and then increases continuously, thus exhibiting a close relationship with the measured alterations in protein synthesis. Another indication of metabolic conversions during regeneration is provided by the changing level of free radioactive leucine, which shows a marked turning point at 24 h after fragmentation. Analysis of free c~-amino concentrations in small regions of larger fragments also indicates the establishment of new intercellular correlations only a few hours after isolation of the cells from the meristem. Up to the 8th h after fragmentation, the contents of both the adaxial and peripheral fragment regions increase. Thereafter only the adaxial (regenerating) cells continue accumulating; the peripheral (nonregenerating) ones remain at the same level.