Early history, discovery, and expression of Aequorea green fluorescent protein, with a note on an unfinished experiment
✍ Scribed by Frederick I. Tsuji
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 589 KB
- Volume
- 73
- Category
- Article
- ISSN
- 1059-910X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The bioluminescent hydromedusan jellyfish, Aequorea
victoria, emits a greenish light (λ~max~ = 508 nm) when stimulated electrically or mechanically. The light comes from photocytes located along the margin of its umbrella. The greenish light depends on two intracellular proteins working in consort: aequorin (21.4 kDa) and a green fluorescent protein (27 kDa). An excited state green fluorescent protein molecule results, which, on returning to the ground state, emits a greenish light. Similarly, a green light emission may be induced in the green fluorescent protein by exposing it to ultraviolet or blue light. Because the green light can be readily detected under a fluorescence microscope, the green fluorescent protein, tagged to a protein of interest, has been used widely as a marker to locate proteins in cells and to monitoring gene expression. This article reviews the work that took place leading to the discovery, cloning, and expression of the green fluorescent protein, with a note on an unfinished experiment. Microsc. Res. Tech. 73:785–796, 2010. © 2010 Wiley‐Liss, Inc.