Early and late transfer of F genes by Hfr donors of E. coli K-12
โ Scribed by Guyer, Mark S. ;Clark, Alvin J.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 780 KB
- Volume
- 157
- Category
- Article
- ISSN
- 0026-8925
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๐ SIMILAR VOLUMES
Using Mu/mini-Mu mixed lysates prepared by induction of Salmonella typhimurium and Klebsiella pneumoniae lysogenic for Mucts62 or the Mu-P1 hybrid, MPh1, and mini-Mu, Mu18A-1, we were able to transfer Arg and His genes from Klebsiella and Salmonella into E. coli K12.
Further understanding of how the FinQ fertility inhibition system of the I-like plasmid R62 inhibits transfer of the sex factor F has been gained by the use of a genetic assay for individual transfer gene products. The technique involved construction of a series of Flac plasmids carrying suppressibl