Early afferent signaling in the outer plexiform layer regulates development of horizontal cell morphology

✍ Scribed by Mary E. Raven; Noelle C. Orton; Hadi Nassar; Gary A. Williams; William K. Stell; Gerald H. Jacobs; N. Torben Bech-Hansen; Benjamin E. Reese

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 463 KB
• Volume: 506
• Category: Article
• ISSN: 0021-9967
• DOI: 10.1002/cne.21615

✦ Synopsis

Abstract

The pedicles of cone photoreceptors, labeled with an antibody to mouse cone arrestin (blue), stratify in the outer plexiform layer, where they form synapses with the dendrites of horizontal and bipolar cells. Those synaptic sites are evidenced by the co‐localization of the synaptic ribbon protein, piccolo (red), with the cone arrestin labeling. The remaining red profiles in the outer plexiform layer indicate the sites of the rod spherules. An antibody to cytochrome oxidase (green) labels the mitochondrion‐rich inner segments of all photoreceptors and yields punctate peri‐nuclear labeling within the outer nuclear layer. Many of these features of the outer retina are altered in the Cacna1f‐mutant retina, expressing a defective calcium channel subunit that prevents normal neurotransmission in the outer plexiform layer. J. Comp. Neurol. 506:745–758, 2007. © 2007 Wiley‐Liss, Inc.