𝔖 Bobbio Scriptorium
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E2 and NS5: New antigens for detection of hepatitis C virus antibodies

✍ Scribed by H. L. Zaaijer; D. S. Vallari; M. Cunningham; R. Lesniewski; H. W. Reesink; C. L. Der Van Poel; P. N. Lelie

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
304 KB
Volume
44
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

The value of two new hepatitis C virus (HCV) antigens for detection of HCV antibodies was studied. These two recombinant antigens were derived from the nonstructural‐5 (NS5) and envelope ‐2 (E2) region of the HCV genome. In a panel of 33 HCV‐RNA positive samples with indeterminate Riba‐2 confirmatory test results, 29 samples (88%) showed additional antibody reactivity against E2 and 12 samples (36%) snowed additional reactivity against NS5. Among 39 HCV‐RNA negative, Riba‐2 indeterminate donor samples, no additional E2 or NS5 reactivity was found in 34 samples (87%); while 5 samples (13%) showed additional reactivity against NS5 and/or E2. E2 reactivity thus resolved the majority of hitherto indeterminate samples. In serial samples from nine posttransfusion hepatitis C patients, NS5 and E2 antibodies did not appear earlier than classical HCV antibodies. However, E2 antibodies eventually appeared in all nine patients. The recombinant E2 might be a candidate antigen for future HCV antibody assays. © 1994 Wiley‐Liss, Inc.

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