E1A-F is overexpressed early in human colorectal neoplasia and associated with cyclooxygenase-2 and matrix metalloproteinase-7
✍ Scribed by William M. Boedefeld II; Richie Soong; Heidi Weiss; Robert B. Diasio; Marshall M. Urist; Kirby I. Bland; Martin J. Heslin
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 97 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0899-1987
- DOI
- 10.1002/mc.20093
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Studies suggest the expression of cyclooxygenase‐2 (COX‐2) and matrilysin (MMP‐7) increase in the early stages of colorectal carcinogenesis, however their interaction with other molecular markers is poorly understood. Results from cell line studies and mouse models suggest polyomavirus enhancer activator 3 (PEA3) may play a role in the activation of COX‐2 and MMP‐7 promoters. However, the role of E1A‐F, the human homolog of murine PEA3, in colorectal cancer (CRC) development has not been elucidated. In this study, we used real‐time reverse transcription (RT)‐polymerase chain reaction (PCR) to measure the levels of E1A‐F, COX‐2, and MMP‐7 in matched normal mucosa, adenomas, and/or carcinomas from 128 patients. Our results demonstrate significant overexpression of E1A‐F and MMP‐7 in adenomas and E1A‐F, COX‐2, and MMP‐7 in carcinomas. In carcinomas, E1A‐F expression was significantly associated with both COX‐2 and MMP‐7 overexpression. These results suggest E1A‐F is overexpressed in early stages of human CRC development and may be an important factor in the overexpression of COX‐2 and MMP‐7. © 2005 Wiley‐Liss, Inc.