E1-Ngn2/Cre is a new line for regional activation of Cre recombinase in the developing CNS
✍ Scribed by Joachim Berger; Silke Eckert; Raffaella Scardigli; François Guillemot; Peter Gruss; Anastassia Stoykova
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 384 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1526-954X
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✦ Synopsis
Abstract
We generated a transgenic mouse line named __E1‐Ngn2/__Cre that expresses Cre recombinase and GFP under the control of the E1 enhancer element of the gene Ngn2 (Scardigli et al.: Neuron 31:203–217, 2001). Cre‐recombinase activity and GFP fluorescence are consistent with the reported expression pattern controlled by the E1‐Ngn2 enhancer. Recombination was detected in the progenitor domains p1 and p2 in the ventricular zone of the neural tube and in distinct domains of the pretectum, the dorsal and ventral thalamus, the tegmentum of the mesencephalon, and the hindbrain. In the developing cortex, Cre‐recombinase activity is confined to a subpopulation of progenitors predominantly in the region of the ventral and lateral pallium. The __E1‐Ngn2/__Cre mouse line thus provides an excellent novel tool for a region‐specific conditional mutagenesis in the developing CNS. genesis 40:195–199, 2004. © 2004 Wiley‐Liss, Inc.