E-LDL and Ox-LDL differentially regulate ceramide and cholesterol raft microdomains in human Macrophages

✍ Scribed by Margot Grandl; Salim Maa Bared; Gerhard Liebisch; Tobias Werner; Stefan Barlage; Gerd Schmitz

• Book ID: 102137981
• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 107 KB
• Volume: 69A
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.a.20232

✦ Synopsis

Abstract

Atherosclerosis is characterized by the generation of lipid‐loaded macrophage‐derived foam cells. To study the effect of different types of atherogenic lipoproteins, human macrophages were loaded with enzymatically degraded low density lipoprotein (E‐LDL) or oxidized LDL (Ox‐LDL). Cellular cholesterol content was increased by E‐LDL, whereas Ox‐LDL increased the ceramide content. Cell surface expression analysis by flow cytometry and confocal microscopy revealed that Ox‐LDL increased ceramide and lactosylceramide expression compared to E‐LDL loading and induced ceramide rafts, whereas loading with E‐LDL induced cholesterol‐rich microdomains. Formation of different rafts may have consequences for raft associated signaling in cholesterol homeostasis and apoptosis in human macrophages. © 2006 International Society for Analytical Cytology