𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Dynamics of single dye molecules observed by confocal imaging and spectroscopy

✍ Scribed by Mark A. Weber; Frank Stracke; Alfred J. Meixner

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
329 KB
Volume
36
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

✦ Synopsis

The fluorescence emission of single rhodamine dye molecules (rhodamine 6G and rhodamine 630) at room temperature was analyzed by using scanning confocal laser microscopy in conjunction with polarization analysis, fluorescence spectroscopy, time-resolved detection (minutes to microseconds), and excitation saturation. Results are presented and discussed 1) for samples with dye molecules at the glass-air interface and 2) covered with an additional thin protective polymer film (polyvinylbutyral). Under the polymer layer, the single-molecule fluorescence was more stable than the glass-air interface. This result may be explained by fewer spontaneous variations of the fluorescence rate, polarization changes, spectral shifts, and longer photochemical lifetimes.

πŸ“œ SIMILAR VOLUMES

Handbook of Fluorescence Spectroscopy an
Handbook of Fluorescence Spectroscopy and Imaging (From Single Molecules to Ensembles) || Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS)
✍ Sauer, Markus; Hofkens, Johan; Enderlein, Jârg πŸ“‚ Article πŸ“… 2011 πŸ› Wiley-VCH Verlag GmbH & Co. KGaA 🌐 German βš– 345 KB πŸ‘ 2 views

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting wi

Handbook of Fluorescence Spectroscopy an
Handbook of Fluorescence Spectroscopy and Imaging (From Single Molecules to Ensembles) || Index
✍ Sauer, Markus; Hofkens, Johan; Enderlein, Jârg πŸ“‚ Article πŸ“… 2011 πŸ› Wiley-VCH Verlag GmbH & Co. KGaA 🌐 German βš– 80 KB πŸ‘ 2 views

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting wi

Handbook of Fluorescence Spectroscopy an
Handbook of Fluorescence Spectroscopy and Imaging (From Single Molecules to Ensembles) || Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways
✍ Sauer, Markus; Hofkens, Johan; Enderlein, Jârg πŸ“‚ Article πŸ“… 2011 πŸ› Wiley-VCH Verlag GmbH & Co. KGaA 🌐 German βš– 139 KB πŸ‘ 2 views

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting wi

Handbook of Fluorescence Spectroscopy an
Handbook of Fluorescence Spectroscopy and Imaging (From Single Molecules to Ensembles) || Basic Principles of Fluorescence Spectroscopy
✍ Sauer, Markus; Hofkens, Johan; Enderlein, Jârg πŸ“‚ Article πŸ“… 2011 πŸ› Wiley-VCH Verlag GmbH & Co. KGaA 🌐 German βš– 325 KB πŸ‘ 2 views

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting wi