Dynamical behavior ofpsbgene transcripts in greening wheat seedlings. I. Time course of accumulation of thepsbAthroughpsbNgene transcripts during light-induced greening
✍ Scribed by Hiroshi Kawaguchi; Isao Fukuda; Takashi Shiina; Yoshinori Toyoshima
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 1002 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
The time course of the accumulation of the transcripts from 13 psb genes encoding a major part of the proteins composing photosystem II during light-induced greening of dark-grown wheat seedlings was examined focusing on early stages of plastid development (0.5 h through 72 h). The 13 genes can be divided into three groups. (1) The psbA gene is transcribed as a single transcript of 1.3 kb in the darkgrown seedlings, but its level increases 5-to 7-fold in response to light due to selective increase in RNA stability as well as in transcription activity. (2)The psbE-F-L-J operon, psbM and psbN genes are transcribed as a single transcript of 1.1 kb, two transcripts of 0.5 and 0.7 kb and a single transcript of 0.3 kb, respectively, in the dark-grown seedlings. The levels of accumulation of every transcript remain unchanged or rather decrease during plastid development under illumination. (3) The psbK-I-D-C gene cluster and psbB-H operon exhibit fairly complicated northern hybridization patterns during the greening process. When a psbC or psbD gene probe was used for northern hybridization, five transcripts differing in length were detected in the etioplasts from 5-day old dark-grown seedlings. After 2 h illumination, two new transcripts of different length appeared. Light induction of new transcripts was also observed in the psbB-H operon.