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Dynamic surface properties of phosphatidylglycerol-dipalmitoylphosphatidylcholine mixed films

โœ Scribed by R.H. Notter; S. Holcomb; R.D. Mavis


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
802 KB
Volume
27
Category
Article
ISSN
0009-3084

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โœฆ Synopsis


This paper studies the dynamic surface pressure-area 0r-A) behavior of phosphatidylglycerol (PG) with a mixed fatty acid distribution (bacterial) in pure and binary mixed films with dipalmitoyl phosphatidylcholine (DPL). At 23ยฐC, bacterial PG frims generate maximum dynamic surface pressures of only 48-49 dyn/cm on a 0.15 M sodium chloride subphase for both dilute and surface excess initial conditions. By contrast, binary mixed films of 90 : 10 DPL/PG reach maximum ~r values of the order of 70 dyn/cm at similar conditions, the same as for pure DPL films. A coRapse plateau ratio criterion is used to show that respreading after dynamic compression past film collapse is enhanced in 90 : 10 DPL/PG films as compared to pure DPL for a dilute surface initial condition, but not for the surface excess condition, at room temperature. Respreading in pure bacterial PG films is also slightly improved over corresponding pure DPL films for some initial conditions at 23ยฐC, but the magnitude of this effect is not as large as might be expected from the significant unsaturated and cycloptopane fatty acid percentage present in bacterial PG. Differential Scanning Calorimetry (DSC) measurements on DPL/PG mixtures show decreasing T c with peak broadening as the percentage of bacterial PG is increased. The experiments here do not establish a clearly required functional role for 10% PG in pulmonary surfactant surface behavior. Further surface studies are suggested before long-term clinical trials of PG containing mixtures for exogenous replacement therapy in Neonatal Respiratory Distress Syndrome (RDS) are initiated on a widespread basis. *A temperature of 35ยฐC or above is implied in the text of the paper of Bangham et al. [8] for the dynamic surface pressure data reported in Fig. 1 of their work. However, maximum 7r-values of ~73 dyn/cm are shown on this figure, which is consistent with room temperature rather than body temperature measurements.


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