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Dynamic Single-molecule Colocalization Imaging - A New Method For Examining Membrane Protein Association In Living Cells

✍ Scribed by McColl, James T.; Alexandre, Ricardo; James, John R.; Dunne, Paul D.; Yoon, Ji Won; Davis, Simon J.; Klenerman, David

Book ID: 119204534
Publisher: Biophysical Society
Year: 2009
Tongue: English
Weight: 37 KB
Volume: 96
Category: Article
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.019

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## Abstract A new algorithm is presented for the detection of single, fluorescence‐labeled proteins in the analysis of images from living cells. It is especially suited for images with just a few (<1 per 10 μm^2^) fluorescence peaks from individual proteins with high background and noise (signal to