Dynamic Monitoring of Total-Body Absorption by 19F NMR Spectroscopy: One Hour Ventilation of HFA-134a in Male and Female Rats
✍ Scribed by John R. Finch; Eric J. Dadey; Stanford L. Smith; Lester I. Harrison; George A. Digenis
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 441 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
Six male and six female Sprague‐Dawley rats were ventilated head‐only for 1 h on a 15% atmosphere of 1, 1, 1, 2‐tetrafluoroethane (HFA‐134a) in air in a magnetic resonance imaging spectrometer. Results from these dynamic ^19^F NMR studies suggest that a steady‐state in vivo concentration of HFA‐134a was approached at approximately 25 min into the exposure. Quantitative integration analysis using an external standard estimated this plateau to be 58.3 ± 11.9 mg of absorbed HFA‐134a per rat. The HFA‐134a ^19^F NMR signal disappeared rapidly following removal of the test atmosphere, with an elimination half‐life of 4.6 ± 0.6 min in the male rats and 4.9 ± 1.5 min in the female rats. The data suggest that there was no statistical difference between the sexes in amount absorbed or in elimination half‐lives.