𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Dynamic light scattering studies of globulins from plant seeds

✍ Scribed by Zirwer, D. ;Gast, K. ;Plietz, P. ;Schlesier, B. ;Schwenke, K. D.

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
220 KB
Volume
30
Category
Article
ISSN
0027-769X

No coin nor oath required. For personal study only.

✦ Synopsis

Dynamic light scattering studies of globulins from plant seeds D. ZIRWER', K. GAST', P. PLIETZ', B. SCHLESIER' and K. D. SCHWENKE? Dynamic light scattering studies were performed on the I IS globulins from Helianthus annuus, from Brussica napus and from Yiciajaba, as well as on the 7s globulin from Phaseolus idgaris. The results for the translational diffusion coefficients, the hydrodynamic Stokes' radii, shape parameters and the solvation of these protein molecules are discussed. It is shown that one is able to obtain realistic values for the solvation of the protein molecules by combining data from dynamic light scattering and small-angle X-ray scattering.

We have been studying for some years plant seed globulins in solution by various methods, including dynamic light scattering (DLS), to get insight into the solution structure of these proteins [1-71. DLS is a very efficient tool to determine hydrodynamic properties of proteins [8, 91. We report here on our results for the 11s globulins from Heliunthus annuus. from Brassicu nupus, and from Viciufubu as well as for the 7s globulin from Phaseolus vulgaris.

πŸ“œ SIMILAR VOLUMES

Internal dynamics of tRNAPhe studied by
Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering
✍ A. Patkowski; W. Eimer; Th. DorfmΓΌller πŸ“‚ Article πŸ“… 1990 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 739 KB

The collective internal dynamics of transfer RNAPhe from brewer's yeast in solution was studied by depolarized dynamic light scattering (DDLS) . Within the melting region of tRNA the depolarized spectra consist of two Lorentzian, where the narrow (slow) component describes the overall rotation of th

Dynamic light-scattering study of semifl
Dynamic light-scattering study of semiflexible polymers: Collagen
✍ Kenji Kubota; Yasunori Tominaga; Satoru Fujime πŸ“‚ Article πŸ“… 1987 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 841 KB

Dynamic light-scattering measurements were carried out for collagen in acetate buffer (pH 4.8) extracted from lathyritic ratskin. The correlation functions were analyzed in terms of the semiflexibility of collagen molecules. The experimental T / K vs K relationship was compared with the theoretical

Dynamic light-scattering study of synthe
Dynamic light-scattering study of synthetic myosin filaments
✍ Noriko Mochizuki-Oda; Satoru Fujime πŸ“‚ Article πŸ“… 1988 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 790 KB

Synthetic myosin filaments of rabbit were prepared. Electron microscopy showed that the number-average length ( L , = 470 nm) and sharpness in length distribution ( L J L , = 1.03,) were independent of ionic strengths of 134, 74, and 44 mM, whereas the number ratio of M-filaments (about 15 nm in dia

Application of dynamic light scattering
Application of dynamic light scattering to studies of protein folding kinetics
✍ Klaus Gast; Gregor Damaschun; Rolf Misselwitz; Dietrich Zirwer πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 722 KB

The applicability of dynamic light scattering to studies of the kinetics of unfolding and refolding reactions of proteins is discussed and demonstrated experimentally. The experimental set-up and the data acquisition and data evaluation schemes that have been optimized for kinetic experiments are de