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Dynamic changes in 13C NMR spectra of intact hearts under conditions of varied metabolite enrichment

✍ Scribed by E. Douglas Lewandowski; Chrys Hulbert

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
279 KB
Volume
19
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Dynamic changes in ^13^C NMR signal from enriched glutamate pools within hearts have been examined under varied conditions of metabolite pool size and fractional enrichment. Relative signal intensities of ^13^C‐enriched glutamate isotope isomers were similar within spectra from both intact hearts and corresponding in vitro samples. The parameters used to assess metabolic activity with ^13^C NMR proved independent of fractional enrichment and pool size. The data show the importance of acknowledging unlabeled, ^13^C NMR invisible metabolites. © 1991 Academic Press, Inc.

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