Dye bleaching and phenol precipitation by phthalic anhydride-modified horseradish peroxidase
✍ Scribed by Anne Marie O'Brien; Ciarán Ó'Fágáin
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2000
- Tongue
- English
- Weight
- 101 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
The phenol precipitation and dye bleaching capabilities of phthalic anhydride±modi®ed horseradish peroxidase C (PA±HRP) were compared with those of native HRP C and ethylene glycolbis-(succinic acid N-hydroxysuccinimide ester)±modi®ed HRP (EG±HRP) reported previously. Removal ef®ciency (percentage of phenol removed from solution under experimental conditions) was determined for native HRP and both modi®ed forms. Removal ef®ciencies at 37 °C were very similar, with b95% removal in each case. Removal ef®ciencies were less at 70 °C overall (range 25±45%) but PA±and EG±HRP removed up to 50% more phenol than native HRP. The three HRP forms showed similar dye bleaching performance at 37 °C in the presence of H 2 O 2 and accelerators (up to 86% colour removal). PA±and EG±HRP showed slightly greater bleaching abilities at 65 °C than native HRP for some of the dye/accelerator combinations tested. Modi®ed HRPs performed better in 40% (v/v) mixtures of dioxane or dimethylformamide.